Acetaminophen Interactions with Phospholipid Vesicles Induced Changes in Morphology and Lipid Dynamics

Acetaminophen (APAP) or paracetamol, despite its wide and common use for pain fever symptoms, shows a variety of side effects, toxic overdose effects. The most form effects APAP is in the liver where phosphatidylcholine major component cell membrane with additional associated functionalities. Although this case, on pure phospholipid membranes have been largely ignored. Here, we used 1,2-di-(octadecenoyl)-sn-glycero-3-phosphocholine (DOPC), commonly found mammalian membranes, to synthesize large unilamellar vesicles investigate how incorporation changes lipid vesicle structure, morphology, fluidity at different concentrations. We combination dynamic light scattering, small-angle neutron X-ray scattering (SANS, SAXS), cryo-TEM structural characterization, spin-echo (NSE) spectroscopy dynamics. showed that bilayer significantly impacts spherical self-assembly terms morphology influences content bilayer, causing decrease bending rigidity. observe number lipids per by almost 28% (0.06 wt % APAP) 19% (0.12 compared DOPC (0 APAP). Our results reduces rigidity 50% into much more irregularly shaped vesicles. structure did not show change when observed SAXS, NSE dynamics addition which causes overall decreased A strong effect tail motion space explored tails increases factor 1.45 (for 0.06 1.75 0.12 without drug.